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diff --git a/academic/MetaPhlAn2/README b/academic/MetaPhlAn2/README new file mode 100644 index 0000000000..15d1bc4265 --- /dev/null +++ b/academic/MetaPhlAn2/README @@ -0,0 +1,36 @@ +MetaPhlAn2 is a computational tool for profiling the composition of +microbial communities (Bacteria, Archaea and Eukaryotes) from meta- +genomic shotgun sequencing data (i.e. not 16S) with species-level. + +With the newly added StrainPhlAn module, it is now possible to perform +accurate strain-level microbial profiling. MetaPhlAn2 relies on ~1.5M +unique clade-specific marker genes. The latest marker information file +`mpa_v29_CHOCOPhlAn_201901_marker_info.txt.bz2` can be found in the +Download page (https://bitbucket.org/biobakery/metaphlan2/downloads/) +identified from ~100,000 reference genomes (~99,500 bacterial and +archaeal and ~500 eukaryotic), allowing: + +* unambiguous taxonomic assignments; +* accurate estimation of organismal relative abundance; +* species-level resolution for bacteria, archaea, eukaryotes and viruses; +* strain identification and tracking +* orders of magnitude speedups compared to existing methods. +* metagenomic strain-level population genomics + +If you use MetaPhlAn2, please cite: +MetaPhlAn2 for enhanced metagenomic taxonomic profiling. Duy Tin Truong, +Eric A Franzosa, Timothy L Tickle, Matthias Scholz, George Weingart, +Edoardo Pasolli, Adrian Tett, Curtis Huttenhower & Nicola Segata. Nature +Methods 12, 902-903 (2015) + +If you use StrainPhlAn, please cite the MetaPhlAn2 paper and the +following StrainPhlAn paper: +Microbial strain-level population structure and genetic diversity from +metagenomes. Duy Tin Truong, Adrian Tett, Edoardo Pasolli, Curtis +Huttenhower, & Nicola Segata. Genome Research 27:626-638 (2017) + +NOTE! +The script places the python files in /usr/share/MetaPhlAn2 and creates +symlinks to /usr/bin. If you are using metaphlan2.py with Ugene, it may +not work with the symlink. Instead, just point Ugene directly to +/usr/share/MetaPhlAn2/metaphlan2.py |